[2018-10-13 01:59:29] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:59:29] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:59:29] Checking for Bowtie index files (genome).. [2018-10-13 01:59:29] Checking for reference FASTA file [2018-10-13 01:59:29] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:59:34] Reading known junctions from GTF file [2018-10-13 01:59:38] Preparing reads left reads: min. length=100, max. length=100, 325143 kept reads (169 discarded) right reads: min. length=100, max. length=100, 324839 kept reads (473 discarded) [2018-10-13 01:59:53] Building transcriptome data files /scratch/8792902.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 02:00:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:08:19] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:08:50] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:09:21] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:09:21] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:09:54] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:10:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:10:13] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:10:22] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:10:32] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:11:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:11:15] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:11:27] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:11:37] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:11:46] Searching for junctions via segment mapping [2018-10-13 02:14:25] Retrieving sequences for splices [2018-10-13 02:16:28] Indexing splices [2018-10-13 02:16:49] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:16:54] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:16:58] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:17:03] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:17:07] Joining segment hits [2018-10-13 02:19:18] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:19:23] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:19:27] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:19:31] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:19:35] Joining segment hits [2018-10-13 02:21:54] Reporting output tracks ----------------------------------------------- [2018-10-13 02:25:04] A summary of the alignment counts can be found in /scratch/8792902.1.linga/tophat2/align_summary.txt [2018-10-13 02:25:04] Run complete: 00:25:35 elapsed