[2018-10-13 01:56:17] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:56:17] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:56:17] Checking for Bowtie index files (genome).. [2018-10-13 01:56:17] Checking for reference FASTA file [2018-10-13 01:56:17] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:56:22] Reading known junctions from GTF file [2018-10-13 01:56:26] Preparing reads left reads: min. length=100, max. length=100, 379600 kept reads (265 discarded) right reads: min. length=100, max. length=100, 379255 kept reads (610 discarded) [2018-10-13 01:56:43] Building transcriptome data files /scratch/8792901.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:57:03] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 02:05:15] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:05:55] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 02:06:36] Resuming TopHat pipeline with unmapped reads [2018-10-13 02:06:36] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:07:19] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:07:29] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:07:43] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:07:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:08:05] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:08:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:09:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:09:15] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:09:27] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:09:38] Searching for junctions via segment mapping [2018-10-13 02:12:25] Retrieving sequences for splices [2018-10-13 02:14:34] Indexing splices [2018-10-13 02:14:57] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:15:01] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:15:06] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:15:10] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:15:14] Joining segment hits [2018-10-13 02:18:17] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:18:21] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:18:26] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:18:30] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:18:34] Joining segment hits [2018-10-13 02:21:03] Reporting output tracks ----------------------------------------------- [2018-10-13 02:24:47] A summary of the alignment counts can be found in /scratch/8792901.1.linga/tophat2/align_summary.txt [2018-10-13 02:24:47] Run complete: 00:28:30 elapsed