[2018-10-13 01:48:09] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:48:09] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:48:09] Checking for Bowtie index files (genome).. [2018-10-13 01:48:09] Checking for reference FASTA file [2018-10-13 01:48:09] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:48:11] Reading known junctions from GTF file [2018-10-13 01:48:13] Preparing reads left reads: min. length=100, max. length=100, 323002 kept reads (203 discarded) right reads: min. length=100, max. length=100, 322463 kept reads (742 discarded) [2018-10-13 01:48:23] Building transcriptome data files /scratch/8792898.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:48:33] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:53:52] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:54:13] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:54:35] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:54:35] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:54:55] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:55:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:55:05] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:55:10] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:55:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:55:36] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:55:40] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:55:46] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:55:51] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:55:57] Searching for junctions via segment mapping [2018-10-13 01:57:22] Retrieving sequences for splices [2018-10-13 01:58:34] Indexing splices [2018-10-13 01:58:46] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:58:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:58:51] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:58:53] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:58:56] Joining segment hits [2018-10-13 02:00:17] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:00:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:00:22] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:00:24] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:00:27] Joining segment hits [2018-10-13 02:01:48] Reporting output tracks ----------------------------------------------- [2018-10-13 02:03:34] A summary of the alignment counts can be found in /scratch/8792898.1.c/tophat2/align_summary.txt [2018-10-13 02:03:34] Run complete: 00:15:24 elapsed