[2018-10-13 01:48:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:48:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:48:25] Checking for Bowtie index files (genome).. [2018-10-13 01:48:25] Checking for reference FASTA file [2018-10-13 01:48:25] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:48:34] Reading known junctions from GTF file [2018-10-13 01:48:39] Preparing reads left reads: min. length=100, max. length=100, 264076 kept reads (171 discarded) right reads: min. length=100, max. length=100, 263814 kept reads (433 discarded) [2018-10-13 01:48:53] Building transcriptome data files /scratch/8792897.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:49:15] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:58:30] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:59:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:59:37] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:59:37] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:00:16] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:00:26] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:00:38] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:00:49] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:01:02] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 02:01:41] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 02:01:52] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 02:02:06] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 02:02:18] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 02:02:28] Searching for junctions via segment mapping [2018-10-13 02:05:10] Retrieving sequences for splices [2018-10-13 02:07:23] Indexing splices [2018-10-13 02:07:45] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:07:49] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:07:54] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:07:58] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:08:02] Joining segment hits [2018-10-13 02:10:21] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:10:26] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:10:30] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:10:35] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:10:40] Joining segment hits [2018-10-13 02:13:00] Reporting output tracks ----------------------------------------------- [2018-10-13 02:16:22] A summary of the alignment counts can be found in /scratch/8792897.1.linga/tophat2/align_summary.txt [2018-10-13 02:16:22] Run complete: 00:27:56 elapsed