[2018-10-13 01:48:26] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:48:26] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:48:26] Checking for Bowtie index files (genome).. [2018-10-13 01:48:26] Checking for reference FASTA file [2018-10-13 01:48:26] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:48:30] Reading known junctions from GTF file [2018-10-13 01:48:35] Preparing reads left reads: min. length=100, max. length=100, 116740 kept reads (62 discarded) right reads: min. length=100, max. length=100, 116660 kept reads (142 discarded) [2018-10-13 01:48:40] Building transcriptome data files /scratch/8792896.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:49:01] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:57:33] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:57:49] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:58:04] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:58:04] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:58:23] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:58:31] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:58:39] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:58:47] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:58:55] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:59:16] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:59:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:59:33] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:59:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:59:49] Searching for junctions via segment mapping [2018-10-13 02:02:15] Retrieving sequences for splices [2018-10-13 02:04:26] Indexing splices [2018-10-13 02:04:48] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:04:52] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:04:56] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:05:00] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:05:04] Joining segment hits [2018-10-13 02:07:19] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 02:07:23] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 02:07:27] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 02:07:31] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 02:07:35] Joining segment hits [2018-10-13 02:09:50] Reporting output tracks ----------------------------------------------- [2018-10-13 02:12:24] A summary of the alignment counts can be found in /scratch/8792896.1.linga/tophat2/align_summary.txt [2018-10-13 02:12:24] Run complete: 00:23:58 elapsed