[2018-10-12 21:26:44] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:26:44] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:26:44] Checking for Bowtie index files (genome).. [2018-10-12 21:26:44] Checking for reference FASTA file [2018-10-12 21:26:44] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:26:46] Reading known junctions from GTF file [2018-10-12 21:26:49] Preparing reads left reads: min. length=100, max. length=100, 208750 kept reads (157 discarded) right reads: min. length=100, max. length=100, 208585 kept reads (322 discarded) [2018-10-12 21:26:55] Building transcriptome data files /scratch/8792753.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:27:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:31:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:32:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:32:17] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:32:17] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:32:31] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:32:35] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:32:41] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:32:45] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:32:50] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:33:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:33:10] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:33:16] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:33:21] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:33:26] Searching for junctions via segment mapping [2018-10-12 21:34:41] Retrieving sequences for splices [2018-10-12 21:35:45] Indexing splices [2018-10-12 21:35:57] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:36:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:36:03] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:36:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:36:08] Joining segment hits [2018-10-12 21:37:20] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:37:22] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:37:26] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:37:28] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:37:31] Joining segment hits [2018-10-12 21:38:42] Reporting output tracks ----------------------------------------------- [2018-10-12 21:40:05] A summary of the alignment counts can be found in /scratch/8792753.1.linga/tophat2/align_summary.txt [2018-10-12 21:40:05] Run complete: 00:13:21 elapsed