[2018-10-13 01:40:43] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:40:43] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:40:43] Checking for Bowtie index files (genome).. [2018-10-13 01:40:43] Checking for reference FASTA file [2018-10-13 01:40:43] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:40:45] Reading known junctions from GTF file [2018-10-13 01:40:48] Preparing reads left reads: min. length=100, max. length=100, 272482 kept reads (196 discarded) right reads: min. length=100, max. length=100, 272233 kept reads (445 discarded) [2018-10-13 01:40:55] Building transcriptome data files /scratch/8792893.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:41:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:45:49] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:46:06] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:46:23] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:46:23] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:46:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:46:45] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:46:50] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:46:55] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:46:59] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:47:16] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:47:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:47:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:47:31] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:47:36] Searching for junctions via segment mapping [2018-10-13 01:48:51] Retrieving sequences for splices [2018-10-13 01:49:57] Indexing splices [2018-10-13 01:50:08] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:50:10] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:50:12] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:50:14] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:50:16] Joining segment hits [2018-10-13 01:51:25] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:51:27] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:51:30] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:51:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:51:34] Joining segment hits [2018-10-13 01:52:44] Reporting output tracks ----------------------------------------------- [2018-10-13 01:54:19] A summary of the alignment counts can be found in /scratch/8792893.1.p16/tophat2/align_summary.txt [2018-10-13 01:54:19] Run complete: 00:13:35 elapsed