[2018-10-13 15:50:05] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:50:05] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:50:05] Checking for Bowtie index files (genome).. [2018-10-13 15:50:05] Checking for reference FASTA file [2018-10-13 15:50:05] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:50:07] Reading known junctions from GTF file [2018-10-13 15:50:09] Preparing reads left reads: min. length=100, max. length=100, 214734 kept reads (96 discarded) right reads: min. length=100, max. length=100, 214635 kept reads (195 discarded) [2018-10-13 15:50:16] Building transcriptome data files /scratch/8793330.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:50:26] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:56:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:56:30] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:56:44] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:56:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:56:54] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:56:57] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:57:02] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:57:06] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:57:10] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:57:20] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:57:24] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:57:28] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:57:32] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:57:36] Searching for junctions via segment mapping [2018-10-13 15:59:13] Retrieving sequences for splices [2018-10-13 16:00:26] Indexing splices [2018-10-13 16:00:37] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:00:40] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:00:42] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:00:45] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:00:47] Joining segment hits [2018-10-13 16:02:06] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 16:02:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 16:02:11] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 16:02:14] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 16:02:16] Joining segment hits [2018-10-13 16:03:35] Reporting output tracks ----------------------------------------------- [2018-10-13 16:05:41] A summary of the alignment counts can be found in /scratch/8793330.1.c/tophat2/align_summary.txt [2018-10-13 16:05:41] Run complete: 00:15:36 elapsed