[2018-10-13 01:31:19] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:31:19] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:31:23] Checking for Bowtie index files (genome).. [2018-10-13 01:31:23] Checking for reference FASTA file [2018-10-13 01:31:23] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:31:30] Reading known junctions from GTF file [2018-10-13 01:31:36] Preparing reads left reads: min. length=100, max. length=100, 343020 kept reads (246 discarded) right reads: min. length=100, max. length=100, 342696 kept reads (570 discarded) [2018-10-13 01:31:51] Building transcriptome data files /scratch/8792888.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:32:12] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:41:23] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:42:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:42:44] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:42:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:43:29] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:43:40] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:43:54] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:44:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:44:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:45:01] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:45:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:45:27] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:45:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:45:51] Searching for junctions via segment mapping [2018-10-13 01:48:37] Retrieving sequences for splices [2018-10-13 01:50:53] Indexing splices [2018-10-13 01:51:16] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:51:20] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:51:25] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:51:30] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:51:35] Joining segment hits [2018-10-13 01:54:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:54:34] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:54:39] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:54:44] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:54:48] Joining segment hits [2018-10-13 01:57:09] Reporting output tracks ----------------------------------------------- [2018-10-13 02:00:43] A summary of the alignment counts can be found in /scratch/8792888.1.linga/tophat2/align_summary.txt [2018-10-13 02:00:43] Run complete: 00:29:24 elapsed