[2018-10-13 01:23:19] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:23:19] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:23:20] Checking for Bowtie index files (genome).. [2018-10-13 01:23:20] Checking for reference FASTA file [2018-10-13 01:23:20] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:23:22] Reading known junctions from GTF file [2018-10-13 01:23:24] Preparing reads left reads: min. length=100, max. length=100, 204162 kept reads (103 discarded) right reads: min. length=100, max. length=100, 203993 kept reads (272 discarded) [2018-10-13 01:23:31] Building transcriptome data files /scratch/8792885.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:23:41] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:28:24] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:28:36] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:28:48] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:28:49] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:29:01] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:29:05] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:29:09] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:29:13] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:29:16] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:29:29] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:29:32] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:29:37] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:29:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:29:45] Searching for junctions via segment mapping [2018-10-13 01:30:58] Retrieving sequences for splices [2018-10-13 01:32:04] Indexing splices [2018-10-13 01:32:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:32:17] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:32:19] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:32:21] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:32:23] Joining segment hits [2018-10-13 01:33:31] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:33:33] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:33:35] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:33:38] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:33:39] Joining segment hits [2018-10-13 01:34:48] Reporting output tracks ----------------------------------------------- [2018-10-13 01:36:13] A summary of the alignment counts can be found in /scratch/8792885.1.p8/tophat2/align_summary.txt [2018-10-13 01:36:13] Run complete: 00:12:53 elapsed