[2018-10-13 01:16:48] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:16:48] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:16:48] Checking for Bowtie index files (genome).. [2018-10-13 01:16:48] Checking for reference FASTA file [2018-10-13 01:16:48] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:16:52] Reading known junctions from GTF file [2018-10-13 01:16:58] Preparing reads left reads: min. length=100, max. length=100, 393524 kept reads (139 discarded) right reads: min. length=100, max. length=100, 393244 kept reads (419 discarded) [2018-10-13 01:17:15] Building transcriptome data files /scratch/8792882.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:17:35] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:25:49] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:26:23] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:26:58] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:26:58] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:27:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:27:52] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:28:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:28:12] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:28:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:29:04] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:29:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:29:24] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:29:34] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:29:43] Searching for junctions via segment mapping [2018-10-13 01:32:15] Retrieving sequences for splices [2018-10-13 01:34:27] Indexing splices [2018-10-13 01:34:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:34:56] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:35:01] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:35:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:35:11] Joining segment hits [2018-10-13 01:37:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:37:35] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:37:40] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:37:44] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:37:49] Joining segment hits [2018-10-13 01:40:16] Reporting output tracks ----------------------------------------------- [2018-10-13 01:43:59] A summary of the alignment counts can be found in /scratch/8792882.1.linga/tophat2/align_summary.txt [2018-10-13 01:43:59] Run complete: 00:27:11 elapsed