[2018-10-13 15:37:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:37:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:37:03] Checking for Bowtie index files (genome).. [2018-10-13 15:37:03] Checking for reference FASTA file [2018-10-13 15:37:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:37:08] Reading known junctions from GTF file [2018-10-13 15:37:12] Preparing reads left reads: min. length=100, max. length=100, 282877 kept reads (195 discarded) right reads: min. length=100, max. length=100, 282351 kept reads (721 discarded) [2018-10-13 15:37:27] Building transcriptome data files /scratch/8793325.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:37:46] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:45:59] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:46:23] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:46:45] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:46:45] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:47:04] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:47:11] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:47:21] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:47:28] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:47:36] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:48:02] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:48:12] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:48:24] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:48:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:48:43] Searching for junctions via segment mapping [2018-10-13 15:51:20] Retrieving sequences for splices [2018-10-13 15:53:29] Indexing splices [2018-10-13 15:53:56] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:54:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:54:05] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:54:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:54:13] Joining segment hits [2018-10-13 15:56:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:56:31] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:56:36] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:56:40] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:56:45] Joining segment hits [2018-10-13 15:59:14] Reporting output tracks ----------------------------------------------- [2018-10-13 16:02:39] A summary of the alignment counts can be found in /scratch/8793325.1.linga/tophat2/align_summary.txt [2018-10-13 16:02:39] Run complete: 00:25:35 elapsed