[2018-10-13 17:26:06] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:26:06] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:26:06] Checking for Bowtie index files (genome).. [2018-10-13 17:26:06] Checking for reference FASTA file [2018-10-13 17:26:06] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:26:08] Reading known junctions from GTF file [2018-10-13 17:26:10] Preparing reads left reads: min. length=100, max. length=100, 207548 kept reads (211 discarded) right reads: min. length=100, max. length=100, 207401 kept reads (358 discarded) [2018-10-13 17:26:16] Building transcriptome data files /scratch/8793396.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:26:26] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:31:14] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:31:27] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:31:40] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:31:40] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:31:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:31:55] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:32:00] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:32:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:32:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:32:20] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:32:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:32:28] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:32:33] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:32:37] Searching for junctions via segment mapping [2018-10-13 17:33:51] Retrieving sequences for splices [2018-10-13 17:34:57] Indexing splices [2018-10-13 17:35:07] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:35:09] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:35:12] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:35:14] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:35:16] Joining segment hits [2018-10-13 17:36:24] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:36:26] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:36:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:36:31] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:36:33] Joining segment hits [2018-10-13 17:37:42] Reporting output tracks ----------------------------------------------- [2018-10-13 17:39:08] A summary of the alignment counts can be found in /scratch/8793396.1.p16/tophat2/align_summary.txt [2018-10-13 17:39:08] Run complete: 00:13:02 elapsed