[2018-10-13 18:32:23] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:32:23] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:32:23] Checking for Bowtie index files (genome).. [2018-10-13 18:32:23] Checking for reference FASTA file [2018-10-13 18:32:23] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:32:28] Reading known junctions from GTF file [2018-10-13 18:32:32] Preparing reads left reads: min. length=100, max. length=100, 533633 kept reads (200 discarded) right reads: min. length=100, max. length=100, 533343 kept reads (490 discarded) [2018-10-13 18:32:55] Building transcriptome data files /scratch/8793441.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:33:14] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:42:32] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:43:14] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:43:57] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:43:57] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:44:40] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:44:50] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:45:02] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:45:14] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:45:24] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:46:06] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:46:16] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:46:30] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:46:43] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:46:56] Searching for junctions via segment mapping [2018-10-13 18:49:57] Retrieving sequences for splices [2018-10-13 18:52:10] Indexing splices [2018-10-13 18:52:36] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:52:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:52:45] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:52:50] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:52:54] Joining segment hits [2018-10-13 18:55:18] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:55:23] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:55:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:55:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:55:37] Joining segment hits [2018-10-13 18:58:13] Reporting output tracks ----------------------------------------------- [2018-10-13 19:01:59] A summary of the alignment counts can be found in /scratch/8793441.1.linga/tophat2/align_summary.txt [2018-10-13 19:01:59] Run complete: 00:29:35 elapsed