[2018-10-13 01:05:25] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 01:05:25] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 01:05:25] Checking for Bowtie index files (genome).. [2018-10-13 01:05:25] Checking for reference FASTA file [2018-10-13 01:05:25] Generating SAM header for Bowtie2Index/genome [2018-10-13 01:05:27] Reading known junctions from GTF file [2018-10-13 01:05:29] Preparing reads left reads: min. length=100, max. length=100, 438553 kept reads (266 discarded) right reads: min. length=100, max. length=100, 438061 kept reads (758 discarded) [2018-10-13 01:05:42] Building transcriptome data files /scratch/8792877.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 01:05:53] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 01:10:41] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:11:06] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 01:11:33] Resuming TopHat pipeline with unmapped reads [2018-10-13 01:11:33] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:12:00] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:12:04] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:12:11] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:12:16] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:12:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 01:12:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 01:12:54] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 01:13:01] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 01:13:06] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 01:13:12] Searching for junctions via segment mapping [2018-10-13 01:14:34] Retrieving sequences for splices [2018-10-13 01:15:41] Indexing splices [2018-10-13 01:15:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:15:54] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:15:57] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:15:59] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:16:01] Joining segment hits [2018-10-13 01:17:16] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:17:19] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:17:21] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:17:24] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:17:26] Joining segment hits [2018-10-13 01:18:41] Reporting output tracks ----------------------------------------------- [2018-10-13 01:20:31] A summary of the alignment counts can be found in /scratch/8792877.1.p8/tophat2/align_summary.txt [2018-10-13 01:20:31] Run complete: 00:15:06 elapsed