[2018-10-13 17:19:19] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:19:19] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:19:19] Checking for Bowtie index files (genome).. [2018-10-13 17:19:19] Checking for reference FASTA file [2018-10-13 17:19:19] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:19:23] Reading known junctions from GTF file [2018-10-13 17:19:27] Preparing reads left reads: min. length=100, max. length=100, 385223 kept reads (194 discarded) right reads: min. length=100, max. length=100, 384904 kept reads (513 discarded) [2018-10-13 17:19:42] Building transcriptome data files /scratch/8793391.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:20:05] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:27:34] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:28:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:28:46] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:28:46] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:29:18] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:29:26] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:29:38] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:29:48] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:29:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:30:29] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:30:37] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:30:50] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:31:00] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:31:10] Searching for junctions via segment mapping [2018-10-13 17:33:59] Retrieving sequences for splices [2018-10-13 17:36:04] Indexing splices [2018-10-13 17:36:25] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:36:29] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:36:34] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:36:38] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:36:42] Joining segment hits [2018-10-13 17:39:02] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:39:06] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:39:11] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:39:15] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:39:20] Joining segment hits [2018-10-13 17:41:39] Reporting output tracks ----------------------------------------------- [2018-10-13 17:45:13] A summary of the alignment counts can be found in /scratch/8793391.1.linga/tophat2/align_summary.txt [2018-10-13 17:45:13] Run complete: 00:25:54 elapsed