[2018-10-13 00:47:22] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 00:47:22] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 00:47:22] Checking for Bowtie index files (genome).. [2018-10-13 00:47:22] Checking for reference FASTA file [2018-10-13 00:47:22] Generating SAM header for Bowtie2Index/genome [2018-10-13 00:47:24] Reading known junctions from GTF file [2018-10-13 00:47:27] Preparing reads left reads: min. length=100, max. length=100, 341838 kept reads (167 discarded) right reads: min. length=100, max. length=100, 341519 kept reads (486 discarded) [2018-10-13 00:47:37] Building transcriptome data files /scratch/8792871.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 00:47:48] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 00:53:39] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:54:00] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:54:22] Resuming TopHat pipeline with unmapped reads [2018-10-13 00:54:22] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:54:46] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:54:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:54:56] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:55:01] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:55:06] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:55:29] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:55:34] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:55:40] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:55:45] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:55:51] Searching for junctions via segment mapping [2018-10-13 00:57:16] Retrieving sequences for splices [2018-10-13 00:58:28] Indexing splices [2018-10-13 00:58:40] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:58:42] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:58:45] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:58:48] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:58:51] Joining segment hits [2018-10-13 01:00:06] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 01:00:08] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 01:00:11] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 01:00:13] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 01:00:15] Joining segment hits [2018-10-13 01:01:31] Reporting output tracks ----------------------------------------------- [2018-10-13 01:03:22] A summary of the alignment counts can be found in /scratch/8792871.1.c/tophat2/align_summary.txt [2018-10-13 01:03:22] Run complete: 00:15:59 elapsed