[2018-10-13 15:23:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:23:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:23:58] Checking for Bowtie index files (genome).. [2018-10-13 15:23:58] Checking for reference FASTA file [2018-10-13 15:23:58] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:24:00] Reading known junctions from GTF file [2018-10-13 15:24:02] Preparing reads left reads: min. length=100, max. length=100, 329405 kept reads (211 discarded) right reads: min. length=100, max. length=100, 329177 kept reads (439 discarded) [2018-10-13 15:24:11] Building transcriptome data files /scratch/8793318.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:24:21] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:29:03] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:29:20] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:29:37] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:29:37] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:29:53] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:29:57] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:30:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:30:07] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:30:11] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:30:27] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:30:32] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:30:37] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:30:42] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:30:46] Searching for junctions via segment mapping [2018-10-13 15:32:10] Retrieving sequences for splices [2018-10-13 15:33:16] Indexing splices [2018-10-13 15:33:27] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:33:29] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:33:31] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:33:34] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:33:36] Joining segment hits [2018-10-13 15:34:45] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:34:47] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:34:49] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:34:52] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:34:54] Joining segment hits [2018-10-13 15:36:04] Reporting output tracks ----------------------------------------------- [2018-10-13 15:38:00] A summary of the alignment counts can be found in /scratch/8793318.1.p16/tophat2/align_summary.txt [2018-10-13 15:38:00] Run complete: 00:14:02 elapsed