[2018-10-13 15:23:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:23:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:23:58] Checking for Bowtie index files (genome).. [2018-10-13 15:23:58] Checking for reference FASTA file [2018-10-13 15:23:58] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:24:00] Reading known junctions from GTF file [2018-10-13 15:24:03] Preparing reads left reads: min. length=100, max. length=100, 295285 kept reads (242 discarded) right reads: min. length=100, max. length=100, 295115 kept reads (412 discarded) [2018-10-13 15:24:11] Building transcriptome data files /scratch/8793317.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:24:22] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:29:04] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:29:20] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:29:37] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:29:37] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:29:48] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:29:52] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:29:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:30:01] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:30:05] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:30:17] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:30:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:30:26] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:30:30] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:30:35] Searching for junctions via segment mapping [2018-10-13 15:32:03] Retrieving sequences for splices [2018-10-13 15:33:09] Indexing splices [2018-10-13 15:33:21] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:33:23] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:33:26] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:33:28] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:33:30] Joining segment hits [2018-10-13 15:34:45] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:34:47] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:34:49] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:34:52] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:34:54] Joining segment hits [2018-10-13 15:36:07] Reporting output tracks ----------------------------------------------- [2018-10-13 15:38:12] A summary of the alignment counts can be found in /scratch/8793317.1.p16/tophat2/align_summary.txt [2018-10-13 15:38:12] Run complete: 00:14:13 elapsed