[2018-10-13 17:13:18] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:13:18] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:13:18] Checking for Bowtie index files (genome).. [2018-10-13 17:13:18] Checking for reference FASTA file [2018-10-13 17:13:18] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:13:21] Reading known junctions from GTF file [2018-10-13 17:13:25] Preparing reads left reads: min. length=100, max. length=100, 344804 kept reads (302 discarded) right reads: min. length=100, max. length=100, 344535 kept reads (571 discarded) [2018-10-13 17:13:39] Building transcriptome data files /scratch/8793389.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:13:56] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:21:47] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:22:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:22:43] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:22:43] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:23:06] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:23:13] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:23:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:23:32] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:23:40] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:24:03] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:24:11] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:24:23] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:24:32] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:24:41] Searching for junctions via segment mapping [2018-10-13 17:27:28] Retrieving sequences for splices [2018-10-13 17:29:37] Indexing splices [2018-10-13 17:29:55] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:30:00] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:30:04] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:30:09] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:30:13] Joining segment hits [2018-10-13 17:32:28] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:32:33] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:32:38] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:32:42] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:32:46] Joining segment hits [2018-10-13 17:34:50] Reporting output tracks ----------------------------------------------- [2018-10-13 17:38:12] A summary of the alignment counts can be found in /scratch/8793389.1.linga/tophat2/align_summary.txt [2018-10-13 17:38:12] Run complete: 00:24:53 elapsed