[2018-10-13 00:14:57] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 00:14:57] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 00:14:57] Checking for Bowtie index files (genome).. [2018-10-13 00:14:57] Checking for reference FASTA file [2018-10-13 00:14:57] Generating SAM header for Bowtie2Index/genome [2018-10-13 00:15:02] Reading known junctions from GTF file [2018-10-13 00:15:07] Preparing reads left reads: min. length=100, max. length=100, 428476 kept reads (215 discarded) right reads: min. length=100, max. length=100, 428219 kept reads (472 discarded) [2018-10-13 00:15:28] Building transcriptome data files /scratch/8792860.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 00:15:48] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 00:24:28] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:25:08] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:25:50] Resuming TopHat pipeline with unmapped reads [2018-10-13 00:25:50] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:26:37] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:26:47] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:27:01] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:27:11] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:27:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:28:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:28:22] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:28:37] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:28:49] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:28:59] Searching for junctions via segment mapping [2018-10-13 00:31:46] Retrieving sequences for splices [2018-10-13 00:33:56] Indexing splices [2018-10-13 00:34:15] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:34:19] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:34:24] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:34:28] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:34:32] Joining segment hits [2018-10-13 00:36:46] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:36:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:36:55] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:36:59] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:37:04] Joining segment hits [2018-10-13 00:39:16] Reporting output tracks ----------------------------------------------- [2018-10-13 00:43:08] A summary of the alignment counts can be found in /scratch/8792860.1.linga/tophat2/align_summary.txt [2018-10-13 00:43:08] Run complete: 00:28:11 elapsed