[2018-10-13 00:11:43] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 00:11:43] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 00:11:43] Checking for Bowtie index files (genome).. [2018-10-13 00:11:43] Checking for reference FASTA file [2018-10-13 00:11:43] Generating SAM header for Bowtie2Index/genome [2018-10-13 00:11:47] Reading known junctions from GTF file [2018-10-13 00:11:52] Preparing reads left reads: min. length=100, max. length=100, 421903 kept reads (294 discarded) right reads: min. length=100, max. length=100, 421495 kept reads (702 discarded) [2018-10-13 00:12:13] Building transcriptome data files /scratch/8792858.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 00:12:34] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 00:21:34] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:22:13] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:22:52] Resuming TopHat pipeline with unmapped reads [2018-10-13 00:22:52] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:23:33] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:23:42] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:23:54] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:24:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:24:11] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:24:52] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:25:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:25:13] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:25:23] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:25:32] Searching for junctions via segment mapping [2018-10-13 00:28:26] Retrieving sequences for splices [2018-10-13 00:30:42] Indexing splices [2018-10-13 00:31:05] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:31:09] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:31:13] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:31:17] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:31:22] Joining segment hits [2018-10-13 00:33:50] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:33:54] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:33:59] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:34:03] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:34:07] Joining segment hits [2018-10-13 00:36:38] Reporting output tracks ----------------------------------------------- [2018-10-13 00:40:51] A summary of the alignment counts can be found in /scratch/8792858.1.linga/tophat2/align_summary.txt [2018-10-13 00:40:51] Run complete: 00:29:08 elapsed