[2018-10-13 15:06:28] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:06:28] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:06:28] Checking for Bowtie index files (genome).. [2018-10-13 15:06:28] Checking for reference FASTA file [2018-10-13 15:06:28] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:06:32] Reading known junctions from GTF file [2018-10-13 15:06:36] Preparing reads left reads: min. length=100, max. length=100, 584406 kept reads (247 discarded) right reads: min. length=100, max. length=100, 584293 kept reads (360 discarded) [2018-10-13 15:07:00] Building transcriptome data files /scratch/8793310.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:07:21] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:15:26] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:16:29] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:17:34] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:17:34] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:18:04] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:18:12] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:18:28] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:18:40] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:18:51] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:19:23] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:19:31] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:19:47] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:19:58] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:20:12] Searching for junctions via segment mapping [2018-10-13 15:25:50] Retrieving sequences for splices [2018-10-13 15:27:51] Indexing splices [2018-10-13 15:28:11] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:28:16] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:28:23] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:28:31] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:28:39] Joining segment hits [2018-10-13 15:31:06] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:31:11] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:31:19] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:31:27] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:31:35] Joining segment hits [2018-10-13 15:34:24] Reporting output tracks ----------------------------------------------- [2018-10-13 15:50:39] A summary of the alignment counts can be found in /scratch/8793310.1.linga/tophat2/align_summary.txt [2018-10-13 15:50:39] Run complete: 00:44:11 elapsed