[2018-10-13 15:03:13] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:03:13] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:03:13] Checking for Bowtie index files (genome).. [2018-10-13 15:03:13] Checking for reference FASTA file [2018-10-13 15:03:13] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:03:15] Reading known junctions from GTF file [2018-10-13 15:03:18] Preparing reads left reads: min. length=100, max. length=100, 752556 kept reads (124 discarded) right reads: min. length=100, max. length=100, 752291 kept reads (389 discarded) [2018-10-13 15:03:37] Building transcriptome data files /scratch/8793308.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:03:47] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:08:29] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:08:59] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:09:28] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:09:28] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:09:45] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:09:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:09:55] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:10:00] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:10:06] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:10:24] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:10:29] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:10:35] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:10:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:10:46] Searching for junctions via segment mapping [2018-10-13 15:12:58] Retrieving sequences for splices [2018-10-13 15:14:04] Indexing splices [2018-10-13 15:14:16] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:14:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:14:22] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:14:25] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:14:27] Joining segment hits [2018-10-13 15:15:43] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:15:45] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:15:49] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:15:51] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:15:54] Joining segment hits [2018-10-13 15:17:10] Reporting output tracks ----------------------------------------------- [2018-10-13 15:21:42] A summary of the alignment counts can be found in /scratch/8793308.1.p16/tophat2/align_summary.txt [2018-10-13 15:21:42] Run complete: 00:18:28 elapsed