[2018-10-13 18:28:11] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 18:28:11] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 18:28:11] Checking for Bowtie index files (genome).. [2018-10-13 18:28:11] Checking for reference FASTA file [2018-10-13 18:28:11] Generating SAM header for Bowtie2Index/genome [2018-10-13 18:28:16] Reading known junctions from GTF file [2018-10-13 18:28:20] Preparing reads left reads: min. length=100, max. length=100, 560918 kept reads (336 discarded) right reads: min. length=100, max. length=100, 560432 kept reads (822 discarded) [2018-10-13 18:28:46] Building transcriptome data files /scratch/8793439.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 18:29:06] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 18:37:18] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:38:01] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 18:38:41] Resuming TopHat pipeline with unmapped reads [2018-10-13 18:38:41] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:39:18] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:39:28] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:39:43] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:39:54] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:40:05] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 18:40:43] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 18:40:55] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 18:41:10] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 18:41:22] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 18:41:34] Searching for junctions via segment mapping [2018-10-13 18:44:31] Retrieving sequences for splices [2018-10-13 18:46:32] Indexing splices [2018-10-13 18:46:51] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:46:55] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:47:00] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:47:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:47:09] Joining segment hits [2018-10-13 18:49:30] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 18:49:35] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 18:49:40] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 18:49:45] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 18:49:49] Joining segment hits [2018-10-13 18:52:05] Reporting output tracks ----------------------------------------------- [2018-10-13 18:55:44] A summary of the alignment counts can be found in /scratch/8793439.1.linga/tophat2/align_summary.txt [2018-10-13 18:55:44] Run complete: 00:27:33 elapsed