[2018-10-13 15:02:03] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 15:02:03] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 15:02:03] Checking for Bowtie index files (genome).. [2018-10-13 15:02:03] Checking for reference FASTA file [2018-10-13 15:02:03] Generating SAM header for Bowtie2Index/genome [2018-10-13 15:02:05] Reading known junctions from GTF file [2018-10-13 15:02:07] Preparing reads left reads: min. length=100, max. length=100, 825855 kept reads (91 discarded) right reads: min. length=100, max. length=100, 825579 kept reads (367 discarded) [2018-10-13 15:02:31] Building transcriptome data files /scratch/8793307.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 15:02:41] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:08:31] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:09:02] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:09:35] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:09:35] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:09:57] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:10:02] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:10:09] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:10:15] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:10:21] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:10:43] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:10:49] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:10:57] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:11:03] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:11:09] Searching for junctions via segment mapping [2018-10-13 15:12:53] Retrieving sequences for splices [2018-10-13 15:14:06] Indexing splices [2018-10-13 15:14:17] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:14:19] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:14:22] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:14:25] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:14:27] Joining segment hits [2018-10-13 15:15:48] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:15:50] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:15:53] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:15:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:15:58] Joining segment hits [2018-10-13 15:17:20] Reporting output tracks ----------------------------------------------- [2018-10-13 15:19:59] A summary of the alignment counts can be found in /scratch/8793307.1.c/tophat2/align_summary.txt [2018-10-13 15:19:59] Run complete: 00:17:56 elapsed