[2018-10-13 14:58:16] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:58:16] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:58:16] Checking for Bowtie index files (genome).. [2018-10-13 14:58:16] Checking for reference FASTA file [2018-10-13 14:58:16] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:58:20] Reading known junctions from GTF file [2018-10-13 14:58:24] Preparing reads left reads: min. length=100, max. length=100, 1294548 kept reads (73 discarded) right reads: min. length=100, max. length=100, 1293936 kept reads (685 discarded) [2018-10-13 14:59:19] Building transcriptome data files /scratch/8793306.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:59:37] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 15:07:08] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:08:02] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 15:08:57] Resuming TopHat pipeline with unmapped reads [2018-10-13 15:08:57] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:09:30] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:09:40] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:09:53] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:10:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:10:15] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 15:10:50] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 15:11:01] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 15:11:14] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 15:11:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 15:11:38] Searching for junctions via segment mapping [2018-10-13 15:15:31] Retrieving sequences for splices [2018-10-13 15:17:44] Indexing splices [2018-10-13 15:18:04] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:18:10] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:18:16] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:18:22] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:18:27] Joining segment hits [2018-10-13 15:20:47] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:20:53] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:21:00] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:21:06] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:21:12] Joining segment hits [2018-10-13 15:23:33] Reporting output tracks ----------------------------------------------- [2018-10-13 15:32:13] A summary of the alignment counts can be found in /scratch/8793306.1.linga/tophat2/align_summary.txt [2018-10-13 15:32:13] Run complete: 00:33:56 elapsed