[2018-10-13 00:01:36] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 00:01:36] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 00:01:36] Checking for Bowtie index files (genome).. [2018-10-13 00:01:36] Checking for reference FASTA file [2018-10-13 00:01:36] Generating SAM header for Bowtie2Index/genome [2018-10-13 00:01:41] Reading known junctions from GTF file [2018-10-13 00:01:45] Preparing reads left reads: min. length=100, max. length=100, 234233 kept reads (199 discarded) right reads: min. length=100, max. length=100, 233981 kept reads (451 discarded) [2018-10-13 00:01:56] Building transcriptome data files /scratch/8792853.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 00:02:15] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 00:10:30] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:10:59] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:11:28] Resuming TopHat pipeline with unmapped reads [2018-10-13 00:11:28] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:12:01] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:12:10] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:12:21] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:12:30] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:12:40] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:13:13] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:13:23] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:13:35] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:13:45] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:13:55] Searching for junctions via segment mapping [2018-10-13 00:16:25] Retrieving sequences for splices [2018-10-13 00:18:32] Indexing splices [2018-10-13 00:18:53] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:18:57] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:19:01] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:19:05] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:19:09] Joining segment hits [2018-10-13 00:21:25] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:21:29] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:21:33] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:21:37] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:21:41] Joining segment hits [2018-10-13 00:23:56] Reporting output tracks ----------------------------------------------- [2018-10-13 00:26:58] A summary of the alignment counts can be found in /scratch/8792853.1.linga/tophat2/align_summary.txt [2018-10-13 00:26:58] Run complete: 00:25:22 elapsed