[2018-10-13 14:46:31] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:46:31] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:46:32] Checking for Bowtie index files (genome).. [2018-10-13 14:46:32] Checking for reference FASTA file [2018-10-13 14:46:32] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:46:35] Reading known junctions from GTF file [2018-10-13 14:46:39] Preparing reads left reads: min. length=100, max. length=100, 1175178 kept reads (166 discarded) right reads: min. length=100, max. length=100, 1174778 kept reads (566 discarded) [2018-10-13 14:47:27] Building transcriptome data files /scratch/8793302.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:47:45] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:55:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:56:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:57:04] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:57:05] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:57:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:57:53] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:58:08] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:58:19] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:58:30] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:59:11] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:59:21] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:59:35] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:59:46] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:59:57] Searching for junctions via segment mapping [2018-10-13 15:03:48] Retrieving sequences for splices [2018-10-13 15:05:52] Indexing splices [2018-10-13 15:06:12] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:06:18] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:06:25] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:06:31] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:06:36] Joining segment hits [2018-10-13 15:09:03] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:09:09] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:09:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:09:22] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:09:28] Joining segment hits [2018-10-13 15:11:53] Reporting output tracks ----------------------------------------------- [2018-10-13 15:20:53] A summary of the alignment counts can be found in /scratch/8793302.1.linga/tophat2/align_summary.txt [2018-10-13 15:20:53] Run complete: 00:34:21 elapsed