[2018-10-13 14:44:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:44:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:44:58] Checking for Bowtie index files (genome).. [2018-10-13 14:44:58] Checking for reference FASTA file [2018-10-13 14:44:58] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:45:00] Reading known junctions from GTF file [2018-10-13 14:45:03] Preparing reads left reads: min. length=100, max. length=100, 668352 kept reads (48 discarded) right reads: min. length=100, max. length=100, 668219 kept reads (181 discarded) [2018-10-13 14:45:21] Building transcriptome data files /scratch/8793301.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:45:31] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:50:13] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:50:37] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:50:59] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:50:59] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:51:13] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:51:18] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:51:23] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:51:28] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:51:33] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:51:46] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:51:50] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:51:56] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:52:00] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:52:05] Searching for junctions via segment mapping [2018-10-13 14:53:49] Retrieving sequences for splices [2018-10-13 14:54:55] Indexing splices [2018-10-13 14:55:06] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:55:08] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:55:11] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:55:13] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:55:15] Joining segment hits [2018-10-13 14:56:29] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:56:32] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:56:34] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:56:37] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:56:40] Joining segment hits [2018-10-13 14:57:54] Reporting output tracks ----------------------------------------------- [2018-10-13 15:01:05] A summary of the alignment counts can be found in /scratch/8793301.1.p16/tophat2/align_summary.txt [2018-10-13 15:01:05] Run complete: 00:16:07 elapsed