[2018-10-13 14:37:50] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:37:50] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:37:50] Checking for Bowtie index files (genome).. [2018-10-13 14:37:50] Checking for reference FASTA file [2018-10-13 14:37:50] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:37:54] Reading known junctions from GTF file [2018-10-13 14:37:58] Preparing reads left reads: min. length=100, max. length=100, 1698159 kept reads (106 discarded) right reads: min. length=100, max. length=100, 1697491 kept reads (774 discarded) [2018-10-13 14:39:09] Building transcriptome data files /scratch/8793299.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:39:28] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:47:34] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:49:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:50:30] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:50:30] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:51:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:51:18] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:51:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:51:44] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:51:57] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:52:42] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:52:53] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:53:09] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:53:23] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:53:37] Searching for junctions via segment mapping [2018-10-13 14:58:31] Retrieving sequences for splices [2018-10-13 15:00:45] Indexing splices [2018-10-13 15:01:07] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:01:14] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:01:22] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:01:30] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:01:37] Joining segment hits [2018-10-13 15:04:01] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:04:08] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:04:16] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:04:26] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:04:34] Joining segment hits [2018-10-13 15:07:42] Reporting output tracks ----------------------------------------------- [2018-10-13 15:24:25] A summary of the alignment counts can be found in /scratch/8793299.1.linga/tophat2/align_summary.txt [2018-10-13 15:24:25] Run complete: 00:46:34 elapsed