[2018-10-13 00:00:00] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 00:00:00] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 00:00:00] Checking for Bowtie index files (genome).. [2018-10-13 00:00:00] Checking for reference FASTA file [2018-10-13 00:00:00] Generating SAM header for Bowtie2Index/genome [2018-10-13 00:00:07] Reading known junctions from GTF file [2018-10-13 00:00:13] Preparing reads left reads: min. length=100, max. length=100, 397999 kept reads (269 discarded) right reads: min. length=100, max. length=100, 397655 kept reads (613 discarded) [2018-10-13 00:00:37] Building transcriptome data files /scratch/8792852.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 00:01:02] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 00:10:15] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:11:00] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 00:11:41] Resuming TopHat pipeline with unmapped reads [2018-10-13 00:11:41] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:12:24] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:12:38] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:12:53] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:13:04] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:13:17] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 00:13:59] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 00:14:13] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 00:14:30] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 00:14:43] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 00:14:57] Searching for junctions via segment mapping [2018-10-13 00:18:48] Retrieving sequences for splices [2018-10-13 00:23:17] Indexing splices [2018-10-13 00:23:42] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:23:47] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:23:55] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:24:01] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:24:08] Joining segment hits [2018-10-13 00:27:22] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 00:27:28] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 00:27:35] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 00:27:40] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 00:27:46] Joining segment hits [2018-10-13 00:31:14] Reporting output tracks ----------------------------------------------- [2018-10-13 00:36:31] A summary of the alignment counts can be found in /scratch/8792852.1.linga/tophat2/align_summary.txt [2018-10-13 00:36:32] Run complete: 00:36:31 elapsed