[2018-10-13 14:28:51] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:28:51] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:28:51] Checking for Bowtie index files (genome).. [2018-10-13 14:28:51] Checking for reference FASTA file [2018-10-13 14:28:51] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:28:53] Reading known junctions from GTF file [2018-10-13 14:28:55] Preparing reads left reads: min. length=100, max. length=100, 230968 kept reads (121 discarded) right reads: min. length=100, max. length=100, 230798 kept reads (291 discarded) [2018-10-13 14:29:02] Building transcriptome data files /scratch/8793291.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:29:13] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:33:55] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:34:10] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:34:25] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:34:25] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:34:40] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:34:44] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:34:49] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:34:53] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:34:58] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:35:12] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:35:17] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:35:22] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:35:26] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:35:31] Searching for junctions via segment mapping [2018-10-13 14:36:48] Retrieving sequences for splices [2018-10-13 14:37:55] Indexing splices [2018-10-13 14:38:07] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:38:09] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:38:11] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:38:14] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:38:16] Joining segment hits [2018-10-13 14:39:25] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:39:27] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:39:29] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:39:31] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:39:34] Joining segment hits [2018-10-13 14:40:43] Reporting output tracks ----------------------------------------------- [2018-10-13 14:42:15] A summary of the alignment counts can be found in /scratch/8793291.1.p16/tophat2/align_summary.txt [2018-10-13 14:42:15] Run complete: 00:13:23 elapsed