[2018-10-13 14:27:34] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:27:34] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:27:34] Checking for Bowtie index files (genome).. [2018-10-13 14:27:34] Checking for reference FASTA file [2018-10-13 14:27:34] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:27:39] Reading known junctions from GTF file [2018-10-13 14:27:43] Preparing reads left reads: min. length=100, max. length=100, 337436 kept reads (85 discarded) right reads: min. length=100, max. length=100, 336913 kept reads (608 discarded) [2018-10-13 14:27:57] Building transcriptome data files /scratch/8793290.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:28:16] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:36:16] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:36:45] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:37:17] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:37:17] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:37:40] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:37:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:37:59] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:38:08] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:38:16] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:38:40] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:38:49] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:39:01] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:39:10] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:39:19] Searching for junctions via segment mapping [2018-10-13 14:42:15] Retrieving sequences for splices [2018-10-13 14:44:21] Indexing splices [2018-10-13 14:44:41] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:44:45] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:44:50] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:44:54] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:44:58] Joining segment hits [2018-10-13 14:47:27] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:47:31] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:47:36] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:47:41] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:47:45] Joining segment hits [2018-10-13 14:50:02] Reporting output tracks ----------------------------------------------- [2018-10-13 14:53:51] A summary of the alignment counts can be found in /scratch/8793290.1.linga/tophat2/align_summary.txt [2018-10-13 14:53:51] Run complete: 00:26:17 elapsed