[2018-10-13 14:32:44] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:32:44] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:32:44] Checking for Bowtie index files (genome).. [2018-10-13 14:32:44] Checking for reference FASTA file [2018-10-13 14:32:44] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:32:46] Reading known junctions from GTF file [2018-10-13 14:32:48] Preparing reads left reads: min. length=100, max. length=100, 2520416 kept reads (608 discarded) right reads: min. length=100, max. length=100, 2519888 kept reads (1136 discarded) [2018-10-13 14:33:51] Building transcriptome data files /scratch/8793294.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:34:09] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:38:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:41:25] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:43:56] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:43:56] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:44:51] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:45:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:45:19] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:45:34] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:45:49] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:46:46] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:46:56] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:47:16] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:47:31] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:47:48] Searching for junctions via segment mapping [2018-10-13 14:58:45] Retrieving sequences for splices [2018-10-13 14:59:52] Indexing splices [2018-10-13 15:00:07] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:00:15] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:00:29] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:00:42] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:00:56] Joining segment hits [2018-10-13 15:02:18] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 15:02:27] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 15:02:42] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 15:02:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 15:03:10] Joining segment hits [2018-10-13 15:04:33] Reporting output tracks ----------------------------------------------- [2018-10-13 15:45:14] A summary of the alignment counts can be found in /scratch/8793294.1.p8/tophat2/align_summary.txt [2018-10-13 15:45:14] Run complete: 01:12:30 elapsed