[2018-10-13 17:03:12] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:03:12] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:03:12] Checking for Bowtie index files (genome).. [2018-10-13 17:03:12] Checking for reference FASTA file [2018-10-13 17:03:12] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:03:16] Reading known junctions from GTF file [2018-10-13 17:03:21] Preparing reads left reads: min. length=100, max. length=100, 187252 kept reads (319 discarded) right reads: min. length=100, max. length=100, 187114 kept reads (457 discarded) [2018-10-13 17:03:29] Building transcriptome data files /scratch/8793384.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:03:48] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:12:02] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:12:26] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:12:50] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:12:50] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:13:10] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:13:19] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:13:30] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:13:40] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:13:50] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:14:11] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:14:20] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:14:32] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:14:41] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:14:51] Searching for junctions via segment mapping [2018-10-13 17:17:15] Retrieving sequences for splices [2018-10-13 17:19:16] Indexing splices [2018-10-13 17:19:37] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:19:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:19:45] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:19:49] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:19:53] Joining segment hits [2018-10-13 17:21:59] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:22:03] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:22:07] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:22:11] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:22:15] Joining segment hits [2018-10-13 17:24:22] Reporting output tracks ----------------------------------------------- [2018-10-13 17:26:59] A summary of the alignment counts can be found in /scratch/8793384.1.linga/tophat2/align_summary.txt [2018-10-13 17:26:59] Run complete: 00:23:46 elapsed