[2018-10-13 14:17:01] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:17:01] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:17:01] Checking for Bowtie index files (genome).. [2018-10-13 14:17:01] Checking for reference FASTA file [2018-10-13 14:17:01] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:17:05] Reading known junctions from GTF file [2018-10-13 14:17:09] Preparing reads left reads: min. length=100, max. length=100, 474748 kept reads (231 discarded) right reads: min. length=100, max. length=100, 474388 kept reads (591 discarded) [2018-10-13 14:17:31] Building transcriptome data files /scratch/8793283.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:17:50] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:25:57] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:26:35] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:27:15] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:27:15] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:27:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:27:50] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:28:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:28:13] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:28:23] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:28:54] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:29:03] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:29:17] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:29:28] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:29:39] Searching for junctions via segment mapping [2018-10-13 14:33:55] Retrieving sequences for splices [2018-10-13 14:36:02] Indexing splices [2018-10-13 14:36:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:36:28] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:36:34] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:36:40] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:36:46] Joining segment hits [2018-10-13 14:39:10] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:39:16] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:39:22] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:39:28] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:39:35] Joining segment hits [2018-10-13 14:42:03] Reporting output tracks ----------------------------------------------- [2018-10-13 14:50:20] A summary of the alignment counts can be found in /scratch/8793283.1.linga/tophat2/align_summary.txt [2018-10-13 14:50:20] Run complete: 00:33:19 elapsed