[2018-10-13 14:04:32] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:04:32] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:04:32] Checking for Bowtie index files (genome).. [2018-10-13 14:04:32] Checking for reference FASTA file [2018-10-13 14:04:32] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:04:34] Reading known junctions from GTF file [2018-10-13 14:04:36] Preparing reads left reads: min. length=100, max. length=100, 1126436 kept reads (210 discarded) right reads: min. length=100, max. length=100, 1126148 kept reads (498 discarded) [2018-10-13 14:05:08] Building transcriptome data files /scratch/8793280.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:05:19] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:10:37] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:11:24] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:12:11] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:12:11] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:12:41] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:12:46] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:12:55] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:13:03] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:13:10] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:13:41] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:13:47] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:13:56] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:14:03] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:14:11] Searching for junctions via segment mapping [2018-10-13 14:17:18] Retrieving sequences for splices [2018-10-13 14:18:31] Indexing splices [2018-10-13 14:18:43] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:18:46] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:18:50] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:18:54] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:18:58] Joining segment hits [2018-10-13 14:20:21] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:20:25] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:20:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:20:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:20:36] Joining segment hits [2018-10-13 14:22:00] Reporting output tracks ----------------------------------------------- [2018-10-13 14:31:16] A summary of the alignment counts can be found in /scratch/8793280.1.c/tophat2/align_summary.txt [2018-10-13 14:31:16] Run complete: 00:26:43 elapsed