[2018-10-13 13:59:18] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:59:18] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:59:18] Checking for Bowtie index files (genome).. [2018-10-13 13:59:18] Checking for reference FASTA file [2018-10-13 13:59:18] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:59:20] Reading known junctions from GTF file [2018-10-13 13:59:22] Preparing reads left reads: min. length=100, max. length=100, 711569 kept reads (74 discarded) right reads: min. length=100, max. length=100, 711352 kept reads (291 discarded) [2018-10-13 13:59:38] Building transcriptome data files /scratch/8793277.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:59:48] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:04:31] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:04:58] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:05:26] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:05:26] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:05:46] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:05:51] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:05:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:06:02] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:06:08] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:06:28] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:06:33] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:06:40] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:06:45] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:06:51] Searching for junctions via segment mapping [2018-10-13 14:08:22] Retrieving sequences for splices [2018-10-13 14:09:29] Indexing splices [2018-10-13 14:09:39] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:09:42] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:09:44] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:09:47] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:09:49] Joining segment hits [2018-10-13 14:11:03] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:11:06] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:11:08] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:11:11] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:11:13] Joining segment hits [2018-10-13 14:12:27] Reporting output tracks ----------------------------------------------- [2018-10-13 14:14:38] A summary of the alignment counts can be found in /scratch/8793277.1.p16/tophat2/align_summary.txt [2018-10-13 14:14:38] Run complete: 00:15:20 elapsed