[2018-10-13 14:16:55] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 14:16:55] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 14:16:55] Checking for Bowtie index files (genome).. [2018-10-13 14:16:55] Checking for reference FASTA file [2018-10-13 14:16:55] Generating SAM header for Bowtie2Index/genome [2018-10-13 14:16:57] Reading known junctions from GTF file [2018-10-13 14:16:59] Preparing reads left reads: min. length=100, max. length=100, 256374 kept reads (20 discarded) right reads: min. length=100, max. length=100, 256288 kept reads (106 discarded) [2018-10-13 14:17:06] Building transcriptome data files /scratch/8793286.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 14:17:17] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 14:22:51] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:23:05] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 14:23:19] Resuming TopHat pipeline with unmapped reads [2018-10-13 14:23:19] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:23:30] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:23:33] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:23:38] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:24:01] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:24:12] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 14:24:26] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 14:24:30] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 14:24:35] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 14:24:40] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 14:24:44] Searching for junctions via segment mapping [2018-10-13 14:26:10] Retrieving sequences for splices [2018-10-13 14:27:22] Indexing splices [2018-10-13 14:27:34] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:27:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:27:39] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:27:41] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:27:43] Joining segment hits [2018-10-13 14:28:57] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:28:59] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:29:02] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:29:04] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:29:06] Joining segment hits [2018-10-13 14:30:20] Reporting output tracks ----------------------------------------------- [2018-10-13 14:32:03] A summary of the alignment counts can be found in /scratch/8793286.1.c/tophat2/align_summary.txt [2018-10-13 14:32:03] Run complete: 00:15:08 elapsed