[2018-10-13 13:37:34] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:37:34] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:37:34] Checking for Bowtie index files (genome).. [2018-10-13 13:37:34] Checking for reference FASTA file [2018-10-13 13:37:34] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:37:36] Reading known junctions from GTF file [2018-10-13 13:37:39] Preparing reads left reads: min. length=100, max. length=100, 1005039 kept reads (193 discarded) right reads: min. length=100, max. length=100, 1004831 kept reads (401 discarded) [2018-10-13 13:38:05] Building transcriptome data files /scratch/8793267.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:38:15] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:42:58] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:43:38] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:44:19] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:44:19] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:44:48] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:44:54] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:45:03] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:45:10] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:45:17] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:45:47] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:45:54] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:46:03] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:46:11] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:46:18] Searching for junctions via segment mapping [2018-10-13 13:50:04] Retrieving sequences for splices [2018-10-13 13:51:10] Indexing splices [2018-10-13 13:51:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:51:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:51:32] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:51:36] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:51:40] Joining segment hits [2018-10-13 13:52:57] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:53:01] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:53:06] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:53:11] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:53:14] Joining segment hits [2018-10-13 13:54:31] Reporting output tracks ----------------------------------------------- [2018-10-13 14:01:46] A summary of the alignment counts can be found in /scratch/8793267.1.p16/tophat2/align_summary.txt [2018-10-13 14:01:46] Run complete: 00:24:11 elapsed