[2018-10-13 13:35:40] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:35:40] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:35:40] Checking for Bowtie index files (genome).. [2018-10-13 13:35:40] Checking for reference FASTA file [2018-10-13 13:35:40] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:35:44] Reading known junctions from GTF file [2018-10-13 13:35:49] Preparing reads left reads: min. length=100, max. length=100, 1401520 kept reads (512 discarded) right reads: min. length=100, max. length=100, 1401180 kept reads (852 discarded) [2018-10-13 13:36:44] Building transcriptome data files /scratch/8793265.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:37:02] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:44:52] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:46:16] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:47:39] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:47:39] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:48:27] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:48:38] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:48:57] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:49:11] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:49:24] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:50:14] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:50:26] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:50:48] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:51:02] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:51:16] Searching for junctions via segment mapping [2018-10-13 13:57:20] Retrieving sequences for splices [2018-10-13 13:59:27] Indexing splices [2018-10-13 13:59:52] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:59:59] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:00:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:00:16] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:00:24] Joining segment hits [2018-10-13 14:03:03] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 14:03:11] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 14:03:20] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 14:03:29] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 14:03:37] Joining segment hits [2018-10-13 14:06:01] Reporting output tracks ----------------------------------------------- [2018-10-13 14:22:56] A summary of the alignment counts can be found in /scratch/8793265.1.linga/tophat2/align_summary.txt [2018-10-13 14:22:56] Run complete: 00:47:16 elapsed