[2018-10-13 13:21:36] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:21:36] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:21:37] Checking for Bowtie index files (genome).. [2018-10-13 13:21:37] Checking for reference FASTA file [2018-10-13 13:21:37] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:21:38] Reading known junctions from GTF file [2018-10-13 13:21:41] Preparing reads left reads: min. length=100, max. length=100, 963315 kept reads (176 discarded) right reads: min. length=100, max. length=100, 963083 kept reads (408 discarded) [2018-10-13 13:22:07] Building transcriptome data files /scratch/8793260.1.c/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:22:18] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:27:49] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:28:36] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:29:23] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:29:23] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:29:53] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:30:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:30:10] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:30:17] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:30:25] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:30:55] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:31:03] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:31:13] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:31:21] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:31:29] Searching for junctions via segment mapping [2018-10-13 13:36:03] Retrieving sequences for splices [2018-10-13 13:37:16] Indexing splices [2018-10-13 13:37:30] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:37:35] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:37:41] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:37:46] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:37:50] Joining segment hits [2018-10-13 13:39:13] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:39:18] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:39:24] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:39:29] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:39:34] Joining segment hits [2018-10-13 13:40:57] Reporting output tracks ----------------------------------------------- [2018-10-13 13:50:24] A summary of the alignment counts can be found in /scratch/8793260.1.c/tophat2/align_summary.txt [2018-10-13 13:50:24] Run complete: 00:28:47 elapsed