[2018-10-12 21:10:14] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:10:14] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:10:14] Checking for Bowtie index files (genome).. [2018-10-12 21:10:14] Checking for reference FASTA file [2018-10-12 21:10:14] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:10:20] Reading known junctions from GTF file [2018-10-12 21:10:25] Preparing reads left reads: min. length=100, max. length=100, 295471 kept reads (223 discarded) right reads: min. length=100, max. length=100, 295191 kept reads (503 discarded) [2018-10-12 21:10:40] Building transcriptome data files /scratch/8792747.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:11:01] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:19:42] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:20:15] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:20:49] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:20:49] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:21:26] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:21:35] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:21:47] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:21:58] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:22:09] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:22:46] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:22:56] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:23:09] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:23:19] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:23:29] Searching for junctions via segment mapping [2018-10-12 21:26:12] Retrieving sequences for splices [2018-10-12 21:28:30] Indexing splices [2018-10-12 21:28:50] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:28:55] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:29:00] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:29:04] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:29:08] Joining segment hits [2018-10-12 21:31:42] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:31:46] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:31:51] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:31:55] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:31:59] Joining segment hits [2018-10-12 21:34:32] Reporting output tracks ----------------------------------------------- [2018-10-12 21:37:53] A summary of the alignment counts can be found in /scratch/8792747.1.linga/tophat2/align_summary.txt [2018-10-12 21:37:53] Run complete: 00:27:38 elapsed