[2018-10-13 13:15:42] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:15:42] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:15:42] Checking for Bowtie index files (genome).. [2018-10-13 13:15:42] Checking for reference FASTA file [2018-10-13 13:15:42] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:15:44] Reading known junctions from GTF file [2018-10-13 13:15:47] Preparing reads left reads: min. length=100, max. length=100, 362882 kept reads (53 discarded) right reads: min. length=100, max. length=100, 362796 kept reads (139 discarded) [2018-10-13 13:15:58] Building transcriptome data files /scratch/8793258.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:16:08] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:21:01] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:21:23] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:21:44] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:21:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:21:56] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:22:00] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:22:05] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:22:09] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:22:14] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:22:25] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:22:28] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:22:33] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:22:38] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:22:43] Searching for junctions via segment mapping [2018-10-13 13:25:03] Retrieving sequences for splices [2018-10-13 13:26:10] Indexing splices [2018-10-13 13:26:23] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:26:25] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:26:28] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:26:31] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:26:35] Joining segment hits [2018-10-13 13:27:48] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:27:51] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:27:53] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:27:56] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:27:59] Joining segment hits [2018-10-13 13:29:13] Reporting output tracks ----------------------------------------------- [2018-10-13 13:33:18] A summary of the alignment counts can be found in /scratch/8793258.1.p8/tophat2/align_summary.txt [2018-10-13 13:33:18] Run complete: 00:17:35 elapsed