[2018-10-13 13:10:58] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 13:10:58] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 13:10:58] Checking for Bowtie index files (genome).. [2018-10-13 13:10:58] Checking for reference FASTA file [2018-10-13 13:10:58] Generating SAM header for Bowtie2Index/genome [2018-10-13 13:11:02] Reading known junctions from GTF file [2018-10-13 13:11:07] Preparing reads left reads: min. length=100, max. length=100, 1062637 kept reads (89 discarded) right reads: min. length=100, max. length=100, 1062453 kept reads (273 discarded) [2018-10-13 13:11:52] Building transcriptome data files /scratch/8793253.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 13:12:11] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:19:51] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:20:46] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:21:41] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:21:41] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:22:21] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:22:30] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:22:46] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:22:57] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:23:08] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:23:49] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:23:58] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:24:14] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:24:25] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:24:36] Searching for junctions via segment mapping [2018-10-13 13:27:57] Retrieving sequences for splices [2018-10-13 13:30:06] Indexing splices [2018-10-13 13:30:27] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:30:31] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:30:37] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:30:41] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:30:46] Joining segment hits [2018-10-13 13:33:17] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:33:22] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:33:28] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:33:32] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:33:37] Joining segment hits [2018-10-13 13:35:56] Reporting output tracks ----------------------------------------------- [2018-10-13 13:42:04] A summary of the alignment counts can be found in /scratch/8793253.1.linga/tophat2/align_summary.txt [2018-10-13 13:42:04] Run complete: 00:31:06 elapsed