[2018-10-12 21:08:41] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:08:41] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:08:41] Checking for Bowtie index files (genome).. [2018-10-12 21:08:41] Checking for reference FASTA file [2018-10-12 21:08:41] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:08:46] Reading known junctions from GTF file [2018-10-12 21:08:51] Preparing reads left reads: min. length=100, max. length=100, 268412 kept reads (157 discarded) right reads: min. length=100, max. length=100, 268197 kept reads (372 discarded) [2018-10-12 21:09:03] Building transcriptome data files /scratch/8792746.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:09:23] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:17:50] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:18:17] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:18:44] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:18:44] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:19:15] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:19:25] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:19:37] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:19:46] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:19:56] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:20:26] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:20:36] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:20:48] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:20:58] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:21:08] Searching for junctions via segment mapping [2018-10-12 21:23:38] Retrieving sequences for splices [2018-10-12 21:25:42] Indexing splices [2018-10-12 21:26:00] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:26:04] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:26:09] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:26:13] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:26:17] Joining segment hits [2018-10-12 21:28:39] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:28:44] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:28:48] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:28:53] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:28:57] Joining segment hits [2018-10-12 21:31:17] Reporting output tracks ----------------------------------------------- [2018-10-12 21:34:18] A summary of the alignment counts can be found in /scratch/8792746.1.linga/tophat2/align_summary.txt [2018-10-12 21:34:18] Run complete: 00:25:36 elapsed