[2018-10-13 17:01:24] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 17:01:24] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 17:01:24] Checking for Bowtie index files (genome).. [2018-10-13 17:01:24] Checking for reference FASTA file [2018-10-13 17:01:24] Generating SAM header for Bowtie2Index/genome [2018-10-13 17:01:28] Reading known junctions from GTF file [2018-10-13 17:01:32] Preparing reads left reads: min. length=100, max. length=100, 439285 kept reads (373 discarded) right reads: min. length=100, max. length=100, 439029 kept reads (629 discarded) [2018-10-13 17:01:50] Building transcriptome data files /scratch/8793382.1.linga/tophat2/tmp/RefSeq_GeneBody [2018-10-13 17:02:09] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 17:10:03] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:10:45] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 17:11:25] Resuming TopHat pipeline with unmapped reads [2018-10-13 17:11:25] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:12:09] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:12:19] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:12:32] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:12:43] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:12:53] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 17:13:38] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 17:13:48] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 17:14:02] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 17:14:13] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 17:14:24] Searching for junctions via segment mapping [2018-10-13 17:17:16] Retrieving sequences for splices [2018-10-13 17:19:15] Indexing splices [2018-10-13 17:19:37] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:19:41] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:19:46] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:19:51] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:19:55] Joining segment hits [2018-10-13 17:22:19] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 17:22:23] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 17:22:29] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 17:22:33] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 17:22:38] Joining segment hits [2018-10-13 17:24:59] Reporting output tracks ----------------------------------------------- [2018-10-13 17:28:54] A summary of the alignment counts can be found in /scratch/8793382.1.linga/tophat2/align_summary.txt [2018-10-13 17:28:54] Run complete: 00:27:29 elapsed