[2018-10-12 21:05:18] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-12 21:05:18] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-12 21:05:19] Checking for Bowtie index files (genome).. [2018-10-12 21:05:19] Checking for reference FASTA file [2018-10-12 21:05:19] Generating SAM header for Bowtie2Index/genome [2018-10-12 21:05:20] Reading known junctions from GTF file [2018-10-12 21:05:23] Preparing reads left reads: min. length=100, max. length=100, 252846 kept reads (190 discarded) right reads: min. length=100, max. length=100, 252664 kept reads (372 discarded) [2018-10-12 21:05:30] Building transcriptome data files /scratch/8792744.1.p16/tophat2/tmp/RefSeq_GeneBody [2018-10-12 21:05:40] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-12 21:10:21] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:10:36] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-12 21:10:50] Resuming TopHat pipeline with unmapped reads [2018-10-12 21:10:50] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:11:04] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:11:08] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:11:12] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:11:16] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:11:20] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-12 21:11:35] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-12 21:11:39] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-12 21:11:44] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-12 21:11:48] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-12 21:11:52] Searching for junctions via segment mapping [2018-10-12 21:13:08] Retrieving sequences for splices [2018-10-12 21:14:15] Indexing splices [2018-10-12 21:14:25] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:14:27] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:14:30] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:14:32] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:14:34] Joining segment hits [2018-10-12 21:15:43] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-12 21:15:45] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-12 21:15:47] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-12 21:15:50] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-12 21:15:52] Joining segment hits [2018-10-12 21:17:01] Reporting output tracks ----------------------------------------------- [2018-10-12 21:18:30] A summary of the alignment counts can be found in /scratch/8792744.1.p16/tophat2/align_summary.txt [2018-10-12 21:18:30] Run complete: 00:13:11 elapsed