[2018-10-13 12:58:26] Beginning TopHat run (v2.1.1) ----------------------------------------------- [2018-10-13 12:58:26] Checking for Bowtie Bowtie version: 2.2.2.0 [2018-10-13 12:58:26] Checking for Bowtie index files (genome).. [2018-10-13 12:58:26] Checking for reference FASTA file [2018-10-13 12:58:26] Generating SAM header for Bowtie2Index/genome [2018-10-13 12:58:28] Reading known junctions from GTF file [2018-10-13 12:58:30] Preparing reads left reads: min. length=100, max. length=100, 445780 kept reads (205 discarded) right reads: min. length=100, max. length=100, 445471 kept reads (514 discarded) [2018-10-13 12:58:42] Building transcriptome data files /scratch/8793248.1.p8/tophat2/tmp/RefSeq_GeneBody [2018-10-13 12:58:52] Building Bowtie index from RefSeq_GeneBody.fa [2018-10-13 13:03:41] Mapping left_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:04:03] Mapping right_kept_reads to transcriptome RefSeq_GeneBody with Bowtie2 [2018-10-13 13:04:26] Resuming TopHat pipeline with unmapped reads [2018-10-13 13:04:26] Mapping left_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:04:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:04:49] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:04:54] Mapping left_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:04:59] Mapping left_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:05:03] Mapping right_kept_reads.m2g_um to genome genome with Bowtie2 [2018-10-13 13:05:23] Mapping right_kept_reads.m2g_um_seg1 to genome genome with Bowtie2 (1/4) [2018-10-13 13:05:27] Mapping right_kept_reads.m2g_um_seg2 to genome genome with Bowtie2 (2/4) [2018-10-13 13:05:33] Mapping right_kept_reads.m2g_um_seg3 to genome genome with Bowtie2 (3/4) [2018-10-13 13:05:38] Mapping right_kept_reads.m2g_um_seg4 to genome genome with Bowtie2 (4/4) [2018-10-13 13:05:42] Searching for junctions via segment mapping [2018-10-13 13:07:17] Retrieving sequences for splices [2018-10-13 13:08:24] Indexing splices [2018-10-13 13:08:34] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:08:36] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:08:39] Mapping left_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:08:41] Mapping left_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:08:43] Joining segment hits [2018-10-13 13:10:02] Mapping right_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie2 (1/4) [2018-10-13 13:10:04] Mapping right_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie2 (2/4) [2018-10-13 13:10:07] Mapping right_kept_reads.m2g_um_seg3 to genome segment_juncs with Bowtie2 (3/4) [2018-10-13 13:10:10] Mapping right_kept_reads.m2g_um_seg4 to genome segment_juncs with Bowtie2 (4/4) [2018-10-13 13:10:12] Joining segment hits [2018-10-13 13:11:28] Reporting output tracks ----------------------------------------------- [2018-10-13 13:13:55] A summary of the alignment counts can be found in /scratch/8793248.1.p8/tophat2/align_summary.txt [2018-10-13 13:13:55] Run complete: 00:15:28 elapsed